Indalo kunye ne-PLoS Iintsholongwane zibonakalisa ubunyani besayensi kwizifundo eziphambili zokudibanisa i-pangolin coronaviruses kwimvelaphi ye-SARS-CoV-2

ushicilelo Imeyli isabelo Ukufuna

Bhalisa ku fumana uhlaziyo kwiBhlogazards Blog.

NguSainath Suryanarayanan, PhD 

Apha, sinika ii-imeyile zethu ngababhali abaphezulu be- Liu okqhubekayo. kwaye Xiao okqhubekayo., kunye nabahleli be Izifo ze-PLoS kwaye indalo. Sikwazisa nengxoxo enzulu yemibuzo kunye neenkxalabo eziphakanyiswe zezi imeyile, ezibeka ukuthandabuza ubunyani bezi zifundo ziphambili ngemvelaphi yenoveli coronavirus SARS-CoV-2 ebangela i-COVID-19. Bona ingxelo yethu kwezi imeyile, Ukunyaniseka kwezifundo eziphambili kwimvelaphi yecoronavirus ngokungathandabuzekiyo; Ijenali zesayensi eziphanda (11.9.20)


Unxibelelwano nge-imeyile noGqirha Jinping Chen, Umbhali omkhulu kaLiu et al:


Ii-imeyile zikaGqirha Jinping Chen ziphakamisa inkxalabo kunye nemibuzo. 

1- Liu okqhubekayo. (2020) bahlanganisa ngokulandelelana kwabo ipangolin coronavirus genome ulandelelwano olusekwe kwii-coronaviruses ezisampuliweyo kwii-pangolin ezintathu, iisampulu ezimbini ezivela kwibhetshi ehanjiswe ngokungekho mthethweni ngo-Matshi 2019, kunye nesampulu enye evela kwibhetshi eyahlukileyo eyabanjwa ngoJulayi 2019. , apho izazinzulu zifuneka ukuba zifake idatha yolandelelwano ukuqinisekisa ukungqinisisa okuzimeleyo kunye nokuzala kwakhona kweziphumo ezipapashiweyo, eziqulathe idatha yolondolozo yokufunda (SRA) ngokulandelelana kweesampulu ezimbini zika-Matshi 2019 kodwa ilahlekile kwidatha kaJulayi 2019. Emva kokubuzwa ngale sampulu ilahlekileyo, ethi uGqirha Jinping Chen ayichaze njenge-F9, uGqirha Jinping Chen wathi: “Idatha eluhlaza yezi sampuli zintathu inokufunyanwa phantsi kwenombolo ye-NCBI ye-PRJNA573298, kunye ne-BioSample ID yayingu-SAMN12809952, SAMN12809953, kunye SAMN12809954, ngaphezu koko, umntu ngamnye (F9) ovela kwibhetshi eyahlukileyo wayenethemba, idatha eluhlaza inokubonwa kwi-NCBI SRA SUB 7661929, eziza kukhutshwa kungekudala ngokuba sinenye i-MS (iphantsi koqwalaselo)”(Ugxininiso lwethu).

Imalunga nelo Liu et al. khange bapapashe idatha engqinelana ne-1 yee-3 zee-pangolins iisampulu abazisebenzisileyo ukudibanisa ukulandelelana kwazo kwe-pangolin coronavirus genome. UGqirha Jinping Chen naye khange abelane ngale datha xa ebuzwa. Isiqhelo kwisayensi kukupapasha kunye / okanye nokwabelana ngedatha eya kuvumela abanye ukuba baqinisekise ngokuzimeleyo kwaye bavelise iziphumo. Ingaba wenze kanjani Izifo ze-PLoS Vumela uLiu et al. ukuphepha ukupapasha idatha yesampulu ebalulekileyo? Kutheni le nto uGqirha Jinping Chen engabelani ngedatha emalunga nesampulu yesithathu yepangolin? Kutheni uLiu et al. Ngaba ufuna ukukhupha idatha engashicilelwanga emalunga nale sampuli yesithathu yepangolin njengenxalenye yesifundo esingeniswe kwiphephancwadi elahlukileyo? Inkxalabo apha yeyokuba izazinzulu ziya kunika gwenxa isampulu yepangolin elahlekileyo evela kuLiu et al. Kwisifundo esahlukileyo, okwenza kube nzima kwabanye ukuba emva koko balandele iinkcukacha ezibalulekileyo malunga nesampulu yepangolin, njengomxholo apho isampulu yepangolin yayiqokelelwa khona.

UGqirha uJinping Chen uyiphikile into yokuba uLiu et al. ndibenolwalamano noXiao et al.'s (2) indalo ukufunda. Ubhale wathi: "Singenise iphepha lethu le-PLOS Pathogens ngoFebruwari 14, 2020 phambi kwephepha leNdalo (Isalathiso 12 kwiphepha lethu le-PLOS pathogens, bangenise ngoFebruwari 16, 2020 ukusukela kumhla wokungeniswa kwabo kwiNdalo), iphepha lethu lezifo ze-PLOS chaza ukuba i-SARS-Cov-2 ayiveli kwipangolin coronavirus ngqo kwaye ipangolin ayingombamkeli ophakathi. Sasiwazi umsebenzi wabo emva kwengcaciso yeendaba zabo ngoFebruwari 7, 2020, kwaye sinezimvo ezahlukileyo kunye nabo, amanye amaphepha amabini (iiVirus kunye neNdalo) adwelisiwe kwiphepha le-PLOS Pathogen njengamaphepha esalathiso (inombolo yesalathiso ye-10 kunye ne-12), singamaqela ophando ahlukeneyo kubabhali bephepha leNdalo, kwaye akukho buhlobo phakathi kwabo, yaye sithathe iisampulu ngolwazi olunenkcukacha zesampula kwiziko lokuhlangula lezilwanyana zasendle laseGuangdong ngoncedo oluvela kuJiejian Zou kunye noFanghui Hou njengababhali bethu kwaye asazi ukuba iisampulu zephepha leNdalo zivela phi. ” (ukunyanzeliswa kwethu)

La manqaku alandelayo aphakamisa ukuthandabuza malunga nezimangalo zikaGqirha Chen ngasentla: 

Liu okqhubekayo. (2020), Xiao et al (2020) kunye noLiu et al. (2019) babelane ngababhali abalandelayo: UPing Liu noJinping Chen babengababhali kwi2019 Iintsholongwane iphepha kunye ne2020 Izifo ze-PLoS iphepha, umbhali omkhulu uWu Chen kwiXiao et al. (2020) wayengumbhali-mbhali we2019 Iintsholongwane iphepha, kunye noJiejian Zhou kunye noFanghui Hou babengababhali kuzo zombini iXiao et al. kunye noLiu et al. 

Zombini ezi mibhalo zibhaliweyo zafakwa kwiseva yoshicilelo loluntu bioRxiv ngomhla ofanayo: ngoFebruwari 20, 2020. 

c Xiao okqhubekayo. “Iisampulu zepangolin ezinikwe igama kwakhona zapapashwa okokuqala nguLiu et al. [2019] Iintsholongwane ngaphandle kokukhankanya isifundo sabo njengenqaku lokuqala elichaze ezi iisampulu, kwaye basebenzise idatha ye-metagenomic kwezi sampuli kuhlalutyo lwabo "(UChan noZhan). 

Liu et al. pangolin coronavirus genome epheleleyo 99.95% efanayo kwinqanaba le-nucleotide ukuya kwi-pangolin coronavirus genome epheleleyo epapashwe ngu-Xiao et al. Angakwazi njani uLiu et al. Ndivelise i-genome epheleleyo eyi-99.95% efanayo (kuphela ~ 15 ye-nucleotides umahluko) kuXiao et al. ngaphandle kokwabelana ngedatha kunye nohlalutyo?

Xa amaqela ophando ohlukeneyo ngokuzimeleyo efika kwiiseti ezifanayo zesigqibo malunga nombuzo onikiweyo wophando, oko kukonyusa ukubakho kwenyani yamabango abandakanyekayo. Inkxalabo apha kukuba uLiu et al. kunye noXiao et al. khange kwenziwe ngokuzimeleyo kwizifundo njengoko kufunwa nguGqirha Chen. Ngaba kukho naluphi na unxibelelwano phakathi kukaLiu et al. kunye noXiao et al. ngokubhekisele kuhlalutyo lwabo nakupapasho? Ukuba kunjalo, lwalungakanani kwaye lwalunjani olo nxibelelaniso? 

Kutheni le nto uLiu et al. ayenzi ukuba ifumaneke esidlangalaleni idatha eluhlaza yokulandelelanisa i-amplicon ababeyisebenzisa ukudibanisa i-pangolin coronavirus genome? Ngaphandle kwale datha eluhlaza, i-pangolin coronavirus genome ehlanganiswe ngu-Liu et al., Abanye abanakuzimela ngokuzimeleyo kwaye bavelise iziphumo zika-Liu et al. Njengoko kukhankanyiwe ngaphambili, into eqhelekileyo kwinzululwazi kukupapasha kunye / okanye nokwabelana ngayo yonke idatha enokuvumela abanye ukuba baqinisekise ngokuzimeleyo kwaye bavelise iziphumo. Sacela uGqirha uJingping Chen ukuba abelane ngeLiu et al. Idatha eluhlaza yokulandelelana kwe-amplicon. Uphendule ngokwabelana no-Liu et al. Iziphumo ze-RT-PCR zokulandelelana kwemveliso, ezingezizo idatha ye-amplicon eluhlaza esetyenziselwa ukudibanisa i-pangolin coronavirus genome. Kutheni uGqirha Jinping Chen emathandabuzo ukukhupha idatha eluhlaza eya kuvumela abanye ukuba baqinisekise ngokuzimeleyo Uhlalutyo lukaLiu et al.

4- Liu okqhubekayo. Iintsholongwane (2019) yapapashwa ngo-Okthobha u-2019 kwaye ababhali bayo babeyifakile i-pangolin coronavirus (ngokulandelelana funda ugcino) idatha ye-SRA kunye ne-NCBI ngoSeptemba 23, 2019, kodwa ndalinda de January 22, 2020 ukwenza le datha ifikeleleke esidlangalaleni. Izazinzulu zihlala zikhupha idatha eluhlaza yokulandelelana kwe-genomic kulwazi olufikeleleka esidlangalaleni ngokukhawuleza emva kokupapashwa kwezifundo zabo. Esi senzo siqinisekisa ukuba abanye banokufikelela ngokuzimeleyo, baqinisekise kwaye basebenzise idatha. Kutheni le nto uLiu et al. 2019 linda iinyanga ezi-4 ukwenza idatha yabo ye-SRA ifikeleleke esidlangalaleni? UGqirha Jinping Chen ukhethe ukungawuphenduli ngokuthe ngqo lo mbuzo wethu kwimpendulo yakhe ngo-Novemba ngo-9, 2020.

Saye sanxibelelana noGqirha Stanley Perlman, Izifo ze-PLoS Umhleli kaLiu et al. kwaye yile nto ebefanele ukuyithetha.

Ngokukodwa, uGqirha Perlman uvumile ukuba:

  • "I-PLoS Pathogens iphanda eli phepha ngokweenkcukacha" 
  • Akazange "aqinisekise ubunyani besampulu kaJulayi 2019 ngexesha lokuphononongwa kwangaphambili koontanga"
  • “[C] ii-oncerns malunga nokufana phakathi kwezi zifundo zibini [Liu et al. noXiao et al.] zavela kuphela emva kokupapashwa kwezi zifundo zombini. ”
  • Akakhange abone nayiphi na idatha ye-amplicon ngexesha lokuphononongwa koontanga. Ababhali banikezele ngenombolo yokungena kwi-genome ehlanganisiweyo… nangona emva kokupapashwa kuye kwavela ukuba inombolo yokungena edweliswe kwiNgxelo yokuFumaneka kweDatha ayichanekanga. Impazamo kunye nemibuzo ejikeleze idatha eluhlaza yokulandelelana kokulungiswa okwangoku kuyaphendulwa njengenxalenye yetyala lasemva kokupapashwa. ”

Xa saqhagamshelana Izifo ze-PLoS neenkxalabo zethu malunga noLiu et al. sikufumene oku kulandelayo Impendulo evela kuMhleli oPhezulu weqela leenqobo ezisisiseko zokupapasha iPLoS:

Iileta ezivela kwiXiao et al.

Ngo-Okthobha i-28, i Umhleli oyiNtloko weSayensi yezebhayoloji indalo uphendule (ngezantsi) ngebinzana eliphambili "siyithatha le micimbi nzulu kwaye siya kuwujonga lo mbandela uwuphakamisa apha ngezantsi ngononophelo olukhulu." 

Nge-30 ka-Okthobha, uXiao et al. ekugqibeleni ikhutshwe esidlangalaleni idatha yabo yokulandelelana kwe-amplicon eluhlaza. Nangona kunjalo, njengopapasho lwesi siqwenga, idatha yolandelelwano lwe-amplicon engeniswe nguXiao et al. ilahlekile ezona fayile zedatha eluhlaza ezinokuvumela abanye ukuba bahlangane kwaye baqinisekise ukulandelelana kwazo kwepangolin coronavirus genome.

Imibuzo ebalulekileyo isasele efuna ukuqwalaselwa: 

  1. Ngaba i-pangolin coronaviruses iyinyani? Isihloko se Umzobo 1e kwiXiao et al. Ithi: “Amasuntswana entsholongwane abonwa kwizicwili ezinamalungu amabini kumfanekiso osasaza i-electron microscopy ethathwe kwiseli yeseli yeVero E6 efakwe isitya esinamandla esingaphezulu kwethishu yamaphaphu evela kwipangolin enye, ne-morphology ebonisa i-coronavirus.” Ukuba uXiao et al. yodwa i-pangolin coronavirus, ngaba bangabelana ngesampulu yentsholongwane kunye nabaphandi abangaphandle kwe China? Oku kunokuhamba indlela ende ekuqinisekiseni ukuba le ntsholongwane ikhona kwaye ivela kwizicwili zepangolin.
  2. Kwangoko ngo-2020, okanye ngo-2019 Liu okqhubekayo., Xiao okqhubekayo., Lam kunye al. kwaye UZhang et al. bayazi ukuba bazakupapasha iziphumo ngokusekwe kwidathasethi efanayo?
    a. Ngaba bekukho naluphi na ulungelelwaniso kuthathelwa ingqalelo ukuba enye yashicilelwa ngo-Februwari 18 kwaye ezintathu zanyulwa kwangaphambili ngo-Februwari 20?
    b. Kutheni le nto uLiu et al. (2019) abenzi ukulandelelana kwabo kufunde idatha yogcino ifikeleleke esidlangalaleni ngomhla ababeyifaka kwiziko ledatha leNCBI? Kutheni le nto belinde kude kube nge-22 kaJanuwari ngo-2020 ukwenza le pangolin coronavirus yokulandelelana kwedatha esidlangalaleni.
    c. Phambi kukaLiu et al. 2019 Iintsholongwane idatha ikhutshwe kwi-NCBI ngoJanuwari 22, 2020, ngaba le datha yayifikeleleka kwabanye abaphandi e-China? Ukuba kunjalo, yeyiphi idathabheyisi eyayiyipangolin coronavirus yokulandelelana kwedatha egcinwe kuyo, ngubani owayenokufikelela, kwaye yafakwa nini idatha yenziwa yafikeleleka?
  3. Ngaba ababhali bazakusebenzisana nophando oluzimeleyo lokulandela umkhondo wezi sampuli zepangolin ukubona ukuba ezinye iintsholongwane ezifana ne-SARS-CoV-2-ezinje ngezo zingafunyanwa ngo-Matshi ukuya ku-Julayi 2019 iibhetshi zezilwanyana ezifihliweyo- ezinokubakho njengeesampulu ezibandayo okanye usaphila eGuangdong Wildlife Rescue Centre?
  4. Ngaba ababhali bazakusebenzisana nophando oluzimeleyo ukubona ukuba abathubelezisi (ngaba bavalelwa?