ʻO ke kūlohelohe a me PLoS Pathogens ʻimi i ka ʻepekema ʻoiaʻiʻo o nā noi nui e pili ana i nā corolavirus pangolin i ke kumu o SARS-CoV-2

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Kākau iā loaʻa nā hōʻike hou mai ka Biohazards Blog.

Na Sainath Suryanarayanan, PhD 

Maʻaneʻi, hāʻawi mākou i kā mākou leka uila me nā mea kākau kiʻekiʻe o Liu et al. a Xiao et al., a me nā luna hoʻoponopono o Nā Polokalamu PLoS a Nature. Hōʻike pū kekahi mākou i kahi kūkākūkā hohonu o nā nīnau a me nā hopohopo i hāpai ʻia e kēia mau leka uila, kahi i kanalua ai i ka pono o kēia mau noiʻi nui e pili ana i ke kumu o ka moʻolelo coronavirus SARS-CoV-2 i kumu ʻo COVID-19. E ʻike i kā mākou hōʻike ʻana i kēia mau leka uila, Ke kūpono o nā noi nui e pili ana i ke kumu o ka coronavirus me ke kānalua; nā puke pai ʻepekema e ʻimi nei (11.9.20)


Nā leka uila me Dr. Jinping Chen, mea kākau kiʻekiʻe o Liu et al:


ʻO nā leka uila a Kauka Jinping Chen e hāpai nei i kekahi mau hopohopo a me nā nīnau: 

1– Liu et al. (2020) hōʻuluʻulu i kā lākou panolin coronavirus genome series i hoʻokumu ʻia e pili ana i nā coronaviruses i ʻāpana ʻia mai ʻekolu mau pangolins, ʻelua mau laʻana mai kahi pūʻulu i hoʻopuka ʻia i Malaki 2019, a me hoʻokahi laʻana mai kahi hui ʻē aʻe i komo ʻia i Iulai 2019. , kahi e koi ʻia ai ka ʻepekema e waiho i ka ʻikepili hoʻonohonoho e hōʻoia i ka hōʻoia kūʻokoʻa a me ka hana hou ʻana o nā hopena i paʻi ʻia, loaʻa ka ʻikepili heluhelu waihona (SRA) ʻikepili no ʻelua mau lā Malaki Malaki 2019 akā ua nalowale nā ​​ʻike no ka laʻana o Iulai 2019. Ma ka nīnau ʻia ʻana e pili ana i kēia hāpana nalo, a Kauka Jinping Chen i hōʻike ai ma ke ʻano he F9, ua ʻōlelo ʻo Kauka Jinping Chen: "Ua ʻike ʻia ka ʻike maka o kēia mau laʻana ʻekolu ma lalo o ka helu helu NCBI PRJNA573298, a ʻo ka ID BioSample ʻo SAMN12809952, SAMN12809953, a SAMN12809954, eia hoʻi, pākahi (F9) mai nā pūʻulu like ʻole he maikaʻi hoʻi, hiki ke ʻike ʻia ka ʻike maka ma NCBI SRA SUB 7661929, e hoʻokuʻu koke ʻia no ka mea he MS hou aʻe kā mākou (ma lalo o ka loiloi)”(Ko mākou koʻikoʻi).

E pili ana ia Liu et al. ʻaʻole i paʻi i ka ʻikepili e pili ana i ka 1 o nā laʻana o 3 pangolins a lākou i hoʻohana ai e hōʻuluʻulu i kā lākou kaʻina genome coronavirus pangolin. ʻAʻole ʻo Kauka Jinping Chen i kaʻanalike i kēia ʻikepili ke nīnau ʻia. ʻO ka maʻamau i ka ʻepekema ka hoʻopuka a / a kaʻana like ʻana i nā ʻikepili āpau e ʻae ai i nā poʻe ʻē aʻe e hōʻoia kūʻokoʻa a hana hou i nā hopena. Pehea Nā Polokalamu PLoS e hoʻokuʻu iā Liu et al. ʻalo i ka paʻi ʻana i ka ʻike ikepili koʻikoʻi? No ke aha ʻo Kauka Jinping Chen e kaʻana ʻole nei i ka ʻikepili e pili ana i kēia hāpana pangolin ʻekolu? No ke aha ʻo Liu et al. makemake e hoʻokuʻu i nā ʻikepili i hoʻopuka ʻole ʻia e pili ana i kēia hāpana pangolin ʻekolu ma ke ʻano he ʻāpana o kahi noi ʻē aʻe i waiho ʻia i kahi puke pai ʻē aʻe? ʻO ka hopohopo ma aneʻi e kuhi hewa nā ʻepekema i ka hāpana pangolin e nalo nei mai Liu et al. i kahi noiʻi ʻokoʻa, e paʻakikī ana i nā poʻe ʻē aʻe e huli hope i nā kikoʻī nui e pili ana i kēia hāpana pangolin, e like me ka pōʻaiapili i ʻohiʻohi ʻia ai ka hāpana pangolin.

2– Ua hōʻole ʻo Kauka Jinping Chen ʻaʻole ʻo Liu et al. ua pili kekahi me Xiao et al.'s (2020) Nature hoʻopaʻa haʻawina. Ua kākau ʻo ia: "Ua hāʻawi mākou i kā mākou PLOS Pathogens pepa ma Pep. 14, 2020 ma mua o ka pepa Nature (ka Reference 12 i kā mākou PLOS pepa pathogens, ua waiho lākou ma Pep. 16, 2020 mai kā lākou lā i waiho ai ma Nature), kā mākou PLOS pathogens pepa. wehewehe ʻaʻole ʻo SARS-Cov-2 mai ka pangolin coronavirus pololei a ʻaʻole ʻo pangolin ma ke ʻano he kikowaena waena. Ua ʻike mākou i kā lākou hana ma hope o kā lākou haʻi ʻōlelo nūhou ma Pepeluali 7, 2020, a he ʻokoʻa ko mākou mau manaʻo me lākou, ua helu ʻia nā pepa ʻē aʻe ʻelua (Virus and Nature) i ka pepa PLOS Pathogen ma ke ʻano he pepa kūmole (helu kuhikuhi 10 a me 12), he mau hui noiʻi ʻokoʻa mākou mai nā mea kākau pepa ʻo Nature, a ʻaʻohe pilina o kekahi me kekahi, a ua lawe mākou i nā mea laʻana me ka ʻike kikoʻī kikoʻī mai ke kikowaena hoʻopakele holoholona ola Guangdong me nā kōkua mai Jiejian Zou a me Fanghui Hou ma ke ʻano he mea kākau pū mākou. a ʻaʻole maopopo iā mākou kahi o nā laʻana o ka pepa Natura. ” (ko mākou koʻikoʻi)

ʻO nā mea aʻe e hāpai nei i nā kānalua no nā ʻōlelo a Kauka Chen ma luna: 

a– Liu et al. (2020), Xiao et al (2020) a me Liu et al. (2019) kaʻana like i kēia mau mea kākau: Ping Liu lāua ʻo Jinping Chen he mau mea kākau ma ka 2019 Nā Virus pepa a me ka 2020 Nā Polokalamu PLoS pepa, mea kākau kiʻekiʻe ʻo Wu Chen ma Xiao et al. (2020) he mea kākau kākau o ka 2019 Nā Virus pepa, a ʻo Jiejian Zhou a me Fanghui Hou nā mea kākau ma Xiao et al. a me Liu et al. 

b– Waiho ʻia nā palapala ʻelua i ka pūnaewele preprint lehulehu bioRxiv ma ka lā like: Pepeluali 20, 2020. 

c– Xiao et al. "Kapa inoa hou ʻia nā laʻana pangolin e Liu et al. [2019] Nā Virus me ka helu ʻole ʻana i kā lākou hoʻopaʻa haʻawina ma ke ʻano he ʻatikala kumu i wehewehe ai i kēia mau laʻana, a hoʻohana i ka ikepili metagenomic mai kēia mau laʻana i kā lākou kālailai ”(ʻO Chan lāua ʻo Zhan). 

d– Liu et al.'s full pangolin coronavirus genome is 99.95% kūlike i ka pae nucleotide a hiki i ka genomine coronaavirus pangolin piha i paʻi ʻia e Xiao et al. Pehea e hiki ai iā Liu et al. ua hana i kahi genome āpau i 99.95% kūlike (ʻokoʻa ~ 15 ʻokoʻa nā nukleotide) iā Xiao et al. me ka kaʻana like ʻole ʻana i nā ʻikepili a me nā kālailai?

Ke hiki kūʻokoʻa i nā pūʻulu noiʻi like ʻole i nā hopena like e pili ana i kahi nīnau noiʻi i hāʻawi ʻia, hoʻonui ia i ka likelike o ka ʻoiaʻiʻo o nā hoʻopiʻi i pili. ʻO ka hopohopo ma aneʻi ʻo Liu et al. and Xiao et al. ʻaʻole alakaʻi kaʻawale ʻia e like me ka ʻōlelo a Dr. Chen. Aia kekahi pilina ma waena o Liu et al. and Xiao et al. e pili ana i kā lākou kālailai a me nā paʻi? Inā pēlā, he aha ka nui a me ke ʻano o kēlā hoʻohui ʻana? 

3– No ke aha ʻo Liu et al. ʻaʻole e hoʻolaha ākea i ka ʻikepili hōʻuluʻulu mana paʻa a lākou i hoʻohana ai e hōʻuluʻulu i ko lākou pangolin coronavirus genome? Me ka ʻole o kēia ʻikepili maka, ʻo ka genolism coronaavirus pangolin i hoʻākoakoa ʻia e Liu et al., ʻAʻole hiki i nā poʻe ʻē aʻe ke hōʻoia kūʻokoʻa a hana hou i nā hopena o Liu et al. E like me ka mea i ʻōlelo ʻia ma mua, ʻo ka maʻamau o ka ʻepekema ka hoʻopuka a / a kaʻana like ʻana i nā ʻikepili āpau e ʻae ai i nā poʻe ʻē aʻe e hōʻoia kūʻokoʻa a hana hou i nā hopena. Ua noi mākou iā Kauka Jingping Chen e kaʻana like i ka ʻike o Liu et al. Ua pane ʻo ia ma ke kaʻana like ʻana i nā hopena o ka huahana RT-PCR a Liu et al. No ke aha e makemake ʻole ai ʻo Kauka Jinping Chen e hoʻokuʻu i ka ʻikepili maka e ʻae ai i nā poʻe ʻē aʻe e hōʻoia kūʻokoʻa iā Liu et al.

4- Liu et al. Nā Virus (2019) i paʻi ʻia ma ʻOkakopa 2019 a ua waiho aku kāna mau mea kākau i kā lākou pangolin coronavirus (waihona heluhelu waihona) ʻikepili SRA me NCBI ma ka lāʻo Ianuali 23, 2019, akā kali a hiki Ianuari 22, 2020 e hoʻike ākea i kēia ʻike. Hoʻokuʻu pinepine nā ʻepekema i ka ʻikepili o ke kaʻina genomic ma luna o ka waihona i hiki i ka lehulehu ke loaʻa koke ma hope o ka paʻi ʻia ʻana o kā lākou noi. Hōʻoia kēia hana i hiki i nā poʻe ʻē aʻe ke komo kūʻokoʻa, hōʻoia a hoʻohana i ia ʻikepili. No ke aha ʻo Liu et al. Kali ʻo 2019 i 4 mau mahina e hiki ai i kā lākou ʻikepili SRA ke ākea ākea? Ua koho ʻo Kauka Jinping Chen ʻaʻole e pane pololei i kēia nīnau a mākou i kāna pane ma Nowemapa 9, 2020.

Ua launa pū mākou me Dr. Stanley Perlman, Nā Polokalamu PLoS Lunahooponopono o Liu et al. a ʻo kēia kāna i ʻōlelo ai.

ʻOiaʻiʻo, ua ʻae ʻo Kauka Perlman e:

  • "Ke noiʻi nei ʻo PLoS Pathogens i kēia pepa i nā kikoʻī hou aʻe" 
  • ʻAʻole ʻo ia i "hōʻoia i ka ʻoiaʻiʻo o ka laʻana o Iulai 2019 i ka wā o ka loiloi mua ʻia e nā hoa"
  • “[C] oncerns e pili ana i ka like ma waena o nā noi ʻelua [Liu et al. a ʻo Xiao et al.] i ʻike ʻia ma hope wale nō o ka paʻi ʻia ʻana o nā noiʻi ʻelua. ”
  • ʻAʻole ʻo ia “ʻike i kekahi ʻikepili amplicon i ka wā o ka loiloi 'ana o nā hoa. Ua hāʻawi nā mea kākau i kahi helu komo no ka genome i ʻākoakoa… ʻoiai ma hope o ka paʻi ʻana ua ʻike ʻia ua hewa ka helu komo i helu ʻia i loko o ka ʻatikala Ka ʻIke ʻIke ʻIke o ka ʻatikala. ʻO kēia kuhi hewa a me nā nīnau e pili ana i ka ʻikepili helu contig sequencing ʻike ʻia i kēia manawa ma ke ʻano he ʻāpana o ka hihia post-publication. "

Ke kāhea aku nei mākou Nā Polokalamu PLoS me ko mākou hopohopo e pili ana iā Liu et al. ua loaʻa iā mākou ka mea aʻe pane mai ka Luna Hooponopono o ka hui PLoS Publication Ethics:

Nā leka uila mai Xiao et al.

Ma ʻOkakopa 28, ka Luna Hooponopono Koiolo Ola o Nature pane (ma lalo) me ka huaʻōlelo kī "lawe mākou i kēia mau pilikia a koʻikoʻi a e nānā pono i ka mea āu e hāpai ai ma lalo nei me ke akahele." 

Ma ʻOkakopa 30, Xiao et al. hope loa hoʻokuʻu ākea ʻO kā lākou ʻikepili moʻo amplicon. Eia nō naʻe, e like me ka paʻi ʻia ʻana o kēia ʻāpana, ua hāʻawi ʻia nā ʻikepili hōʻuluʻulu mana e Xiao et al. ke nalo nei i nā faile ʻike maka maoli e ʻae ai i nā poʻe ʻē aʻe e ʻākoakoa a hōʻoia i kā lākou kaʻina genome virus coronavirus pangolin.

ʻO nā nīnau nui i koe e pono e kamaʻilio ʻia. 

  1. He ʻoiaʻiʻo nā coronaviruses pangolin? ʻO ka lepe kiʻi no Hōʻike 1e ma Xiao et al. ʻ stateslelo: "ʻIke ʻia nā huehue Viral i nā vesicle membrane pālua i ke kiʻi microscopy electron hoʻoili i lawe ʻia mai ka moʻomeheu cell Vero E6 i hoʻopili ʻia me ka supernatant o nā kiko o ka homogenized lung mai hoʻokahi pangolin, me ka morphology e hōʻike ana i ka coronavirus." Inā Xiao et al. hoʻokaʻawale i ka coronavirus pangolin, e kaʻana like lākou i ka hāpana virus i hoʻokaʻawale ʻia me nā mea noiʻi ma waho o Kina? Hiki i kēia ke hele ma kahi lōʻihi loa i ka hōʻoia ʻana e kū nei kēia maʻi a hele mai mai nā aʻa pangolin.
  2. Pehea ka mua o 2020, a ʻo 2019 paha Liu et al., Xiao et al., Lam et al. a Zhang et al. e hoʻomaopopo nei e paʻi ana lākou i nā hopena e pili ana i ka ʻikepili like.
    a. Aia kekahi hoʻonohonoho e noʻonoʻo nei i paʻi ʻia kekahi ma Pepeluali 18 a ʻekolu i paʻi ʻia ma Pepeluali 20?
    b. No ke aha ʻo Liu et al. (2019) mai hana i kā lākou kaʻina heluhelu i ka ʻikepili archive ākea ākea ma ka lā a lākou i waiho ai ma ka NCBI database? No ke aha lākou i kali ai a hiki i Ianuali 22, 2020 e hoʻolaha i kēia ʻikepili kaʻina coronavirus pangolin.
    c. Ma mua o ka Liu et al. 2019 Nā Virus ua hoʻokuʻu ʻia ka ʻike ma NCBI ma Ianuali 22, 2020, ua hiki i kēia ʻikepili i nā kānaka noiʻi ʻē aʻe ma Kina? Inā pēlā, he aha ka pūnaewele i hoʻopaʻa ʻia ai ka ʻikepili ma ka pangolin coronavirus, a ʻo wai ka loaʻa, a i ka manawa hea i waiho ʻia ai ka ʻikepili a hiki ke maʻalahi ʻia?
  3. E hui pū anei nā mea kākau i kahi hoʻokolokolo kūʻokoʻa e nānā i ke kumuwaiwai o kēia mau hiʻohiʻona pangolin e ʻike inā hiki ke ʻike ʻia nā virus hou aʻe e like me ka SARS-CoV-2 i loko o Malaki a Iulai Iulai ke ola nei nō ia ma ka Guangdong Wildlife Rescue Center?
  4. A e hui pū paha nā mea kākau i kahi hoʻokolokolo kūʻokoʻa e ʻike ai inā loaʻa nā mea hoʻopuka pōpilikia (i hoʻopaʻa ʻia lākou i ka hale paʻahao? A i ʻole i hoʻopaʻi ʻia a hoʻokuʻu ʻia?) I loaʻa nā mea ʻino i ka maʻi SARS mai ka hōʻike mau ʻana i kēia mau maʻi?